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🟡 DOI: 10.1111/aor.13518
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Abstract
Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.
Authors
- Assal Daneshgar
- Peter Tang
- Christopher Remde
- Michael Lommel
- Simon Moosburner
- Ulrike Kertscher
- Oliver Klein
- Marie Weinhart
- Johann Pratschke
- Igor M. Sauer
- Karl H. Hildebrandt